Authors
Citation
Sb. Hong et Fm. Raushel, SYNTHESIS AND ENZYMATIC-HYDROLYSIS OF A LIGHT-EMITTING SUBSTRATE FOR PHOSPHOTRIESTERASE, Bioorganic & medicinal chemistry letters, 4(22), 1994, pp. 2705-2708
Citations number
6
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
ISSN journal
0960894X
Volume
4
Issue
22
Year of publication
1994
Pages
2705 - 2708
Database
ISI
SICI code
0960-894X(1994)4:22<2705:SAEOAL>2.0.ZU;2-H
Abstract
The diethylphosphate derivative of hydroxyphenyl)spiro[1,2-dioxetane-3
,2'-adamantane] was synthesized. The K-m and V-max values for the enzy
matic hydrolysis by phosphotriesterase were 0.091 mM and 0.15 sec(-1),
respectively. The enzyme-catalyzed hydrolysis was accompanied by the
emission of visible light.