STUDY OF EFFICACIES OF LEUKOCYTE-DEPLETED TERMINAL BLOOD CARDIOPLEGIAIN 24-HOUR PRESERVED HEARTS

To evaluate the effect of leukocyte-depleted terminal blood cardiopleg ia on prolonged preservation, 41 canine hearts were stored in modified Collins' solution and transplanted heterotopically. Hearts were trans planted soon after harvesting in group 1 and after 24-hour preservatio n in groups 2, 3, and 4. Blood cardioplegia was applied just before ao rtic unclamping in groups 3 and 4; group 3 received simple blood cardi oplegia and group 4 received leukocyte-depleted cardioplegia. The perc entage of the preload recruitable stroke work and diastolic compliance after transplantation compared with the preharvesting value in group 4 did not differ from those in group 1, but the percentage of the prel oad recruitable stroke work in groups 2 and 3 was significantly lower than that in groups 1 and 4. The percentage of diastolic compliance in groups 2 and 3 was significantly higher than that in groups 1 and 4. Coronary blood now 40 minutes after aortic unclamping in group 4 did n ot differ from that in group 1, but was significantly higher than the blood flows in groups 2 and 3. Significant production of malondialdehy de was detected during terminal blood cardioplegia and 10 minutes afte r aortic unclamping in groups 2 and 3, but never in groups 1 and 4. Af ter leukocyte-depleted terminal cardioplegia, the myocardial adenosine triphosphate content increased to the preharvesting value in group 4. Our results suggest that leukocyte-depleted terminal blood cardiopleg ia may be effective in replenishing the energy-depleted myocardium and reducing reperfusion injury, resulting in adequate cardiac function.