The redox conversion of heme-containing protein horseradish peroxidase
(HRP), which has a molar mass of 40,000, was studied. The conversion
was obtained at an electrochemical polymerized o-phenylenediamine (PPD
) film-modified platinum electrode. Optical chin-laver spectroelectroc
hemical techniques were applied to determine the characteristics of th
e electrochemical processes. The modified film can mediate the redox c
onversion of the HRP.