DESICCATION AND CRYOPRESERVATION OF OLIVE (OLEA-EUROPAEA L) EMBRYOS

Authors
GONZALEZRIO F GURRIARAN MJ GONZALEZBENITO E REVILLA MA
Citation
F. Gonzalezrio et al., DESICCATION AND CRYOPRESERVATION OF OLIVE (OLEA-EUROPAEA L) EMBRYOS, Cryo-letters, 15(6), 1994, pp. 337-342
Citations number
15
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
15
Issue
6
Year of publication
1994
Pages
337 - 342
Database
ISI
SICI code
0143-2044(1994)15:6<337:DACOO(>2.0.ZU;2-J
Abstract
Storage of olive fruits at 4 degrees C resulted in a progressive decre ase in the germination capacity of in vitro cultured excised embryos. To prevent this viability loss during storage a cryopreservation proce dure was developed for isolated embryos. High germination percentages (70%) were obtained after rapid immersion of desiccated embryos (3-10% moisture content) in liquid nitrogen. Morphologically uniform plants were regenerated from cryopreserved embryos.