CHANGES IN PEROXIDASE-ACTIVITY IN BEAN SUSPENSION-CULTURES AFTER B-CINEREA AND ELICITOR TREATMENT

Treatment of cell suspension cultures of bean (Phaseolus vulgaris) c.v . 'Gold Saxa') with Botrytis cinerea resulted in the increase in extra - and intracellular peroxidase activity. A similar effect was obtained by treatment of cell suspension cultures with an elicitor active extr act from fungal mycelium. It was shown by means of electrofocusing tha t the infection- or elicitor-related increase in total intracellular p eroxidase activity was associated with the increase in the activity of a specific isozyme with an isoelectric point of 4.8. This anionic per oxidase did not differ in substrate specificity to guaiacol, syringald azine and chlorogenic acid.