LABELING OF DOUBLE-STRANDED DNA BY ROX-DIDEOXYCYTOSINE TRIPHOSPHATE USING TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND SEPARATION BY CAPILLARYELECTROPHORESIS

Terminal transferase is used to add a single fluorescently labeled did eoxynucleotide to double-stranded DNA prepared by restriction endonucl ease action on a bacteriophage. The product is separated by capillary electrophoresis with both hydroxypropylmethylcellulose and non-crossli nked polyacrylamide. The reaction products generate single peaks for e ach fragment with hydroxypropylmethylcellulose. However, the higher re solution separation produced by non-cross-linked polyacrylamide shows that the product contains two components for each restriction digest f ragment. This labeling technique should be useful in restriction fragm ent length polymorphism studies.