DEFINITION OF A SEQUENCE-TAGGED SITE FOR THE HUMAN-IMMUNOGLOBULIN IGLV9S1 GENE LOCATED AT CHROMOSOME 22Q11

We have compared the sequences of the nine human immunoglobulin V-lamb da gene subgroups in order to define specific sequences for the IGLV9S I gene. The oligonucleotides corresponding to these regions, in both f orward and reverse positions, were used in polymerase chain reactions from human genomic DNA. A unique fragment of 177 base pairs was amplif ied from positions 136-312 of the IGLV9S1 gene and cloned in pUC18. Wh en used as a probe in Southern hybridization with human genomic DNA, a unique band was detected, indicating that IGLV9S1 is a single-copy ge ne. We have defined this fragment as a sequence-tagged site designated IGLV9S1[/22q11] for the IGL locus.