HUMAN-IGG SUBCLASS RESPONSES AND SUBCLASS RESTRICTION TO SCHISTOSOMA-MANSONI EGG ANTIGENS

In areas endemic for schistosomiasis, there is great heterogeneity in antibody isotype responses to parasite antigens amongst infected indiv iduals. At the population level, the isotype composition of antibody r esponses undergoes dynamic changes which are associated with the age o f infected individuals. Here we examine the IgG subclass responses to Schistosoma mansoni eggs (soluble egg antigens; SEA) of infected indiv iduals by immunoblot and ELISA. By controlled treatment of SEA-coated ELISA plates and immunoblot nitrocellular strips with sodium periodate , in order to oxidize terminal carbohydrate residues selectively, we w ere able to relate individuals subjects' isotype responses to the diff erent antigens that they responded to, and to the presence of putative carbohydrate and peptide epitopes on those antigens. IgG2 responses w ere restricted strictly to sodium periodate-sensitive carbohydrate epi topes and antigens of relatively high molecular weight. These antigens were not usually recognized by other isotypes and, therefore, they we re only recognized by individuals who had high levels of IgG2. IgG1 an d IgG3 responses were directed against both carbohydrate and peptide e pitopes, whereas IgG4 responses were restricted to periodate-resistant epitopes. This suggests that the fall in IgG2 responses, and reciproc al rise in IgG4 antibodies, seen in young children as their intensitie s of schistosome infection increase, is not the result of isotype swit ching, and that, if these two subclasses are involved in blocking immu nity to schistosomiasis, they are operating independently.