T(H)1-LIKE HUMAN T-CELL CLONES RECOGNIZING LEISHMANIA GP63 INHIBIT LEISHMANIA-MAJOR IN HUMAN MACROPHAGES

Citation
M. Kemp et al., T(H)1-LIKE HUMAN T-CELL CLONES RECOGNIZING LEISHMANIA GP63 INHIBIT LEISHMANIA-MAJOR IN HUMAN MACROPHAGES, Scandinavian journal of immunology, 40(6), 1994, pp. 629-635
Citations number
40
Categorie Soggetti
Immunology
ISSN journal
03009475
Volume
40
Issue
6
Year of publication
1994
Pages
629 - 635
Database
ISI
SICI code
0300-9475(1994)40:6<629:THTCRL>2.0.ZU;2-9
Abstract
The major surface protease of Leishmania major, gp63, has been suggest ed as a vaccine candidate for cutaneous leishmaniasis. In this study g p63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who ha d previously had self-healing cutaneous leishmaniasis. The T-cell clon es expressed CD4, and the alpha chain of the T-cell antigen receptor. Gp63 reactive T-cell clones activated by antigen or by immobilized ant i-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling T(h)1 cells. Autologou s mononuclear cells and Epstein-Barr virus-transformed B cell lines we re equally efficient in presenting the antigen to the T cells. The gp6 3 reactive T cells induced resistance to infection in cultured human m acrophages by L. major. The data confirm that human CD4(+) T cells rec ognizing gp63 can take part in the host defence against L. major infec tions.