CREATION OF AN INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CA2-PRODUCING CELLS( STORE IN SECRETORY GRANULES OF INSULIN)

Authors
BLONDEL O BELL GI MOODY M MILLER RJ GIBBONS SJ
Citation
O. Blondel et al., CREATION OF AN INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CA2-PRODUCING CELLS( STORE IN SECRETORY GRANULES OF INSULIN), The Journal of biological chemistry, 269(44), 1994, pp. 27167-27170
Citations number
23
Categorie Soggetti
Biology
Journal title
The Journal of biological chemistry → ACNP
ISSN journal
00219258
Volume
269
Issue
44
Year of publication
1994
Pages
27167 - 27170
Database
ISI
SICI code
0021-9258(1994)269:44<27167:COAI1C>2.0.ZU;2-1
Abstract
A rapid rise in the cytosolic free Ca2+ concentration due to influx of extracellular Ca2+ or mobilization from intracellular stores is the p rimary trigger for exocytosis from secretory cells. Our understanding as to the precise role of Ca2+ mobilization has been complicated by th e presence of several types of Ca2+ stores in most cells. We now demon strate that overexpression of the type 3 inositol 1,4,5-trisphosphate (IP2) receptor in insulin-secreting beta TC-3 cells results in the cre ation of a unique IP3-sensitive Ca2+ pool, restricted to the insulin s ecretory granules of these cells.