INHERITANCE OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AND RANDOM AMPLIFIED POLYMORPHIC DNAS IN COASTAL DOUGLAS-FIR

A total of 225 new genetic loci [151 restriction fragment length polym orphisms (RFLP) and 74 random amplified polymorphic DNAs (RAPD)] in co astal Douglas-fir [Pseudotsuga menziesii (Mirb.) France var. menziesii ] have been identified using a three-generation outbred pedigree. The Mendelian inheritance of 16 RFLP loci and 29 RAPD loci was demonstrate d based on single-locus segregation in a sample of F-2 progeny. One RF LP locus, PtIFG2025, showed segregation distortion. Probe pPtIFG2025 i s a loblolly pine cDNA probe encoding for rbcS. The 16 RFLP loci and 2 3 allozyme loci were also assayed in a sample of 16 Douglas-fir seed-o rchard clones. Allelism was determined at 11 of the 16 RFLP loci. RFLP s were able to detect slightly more variation (4.0 alleles per locus) than allozymes (3.1 alleles per locus). The inheritance of an addition al 80 RAPD loci was determined based on haploid segregation analysis o f megagametophytes from parent tree 013-1. Once 200-300 markers are id entified and placed on a genetic map, quantitative trait loci affectin g bud phenology will be mapped.