APPLICATION OF CYCLODEXTRIN TO MICROBIAL TRANSFORMATION OF VITAMIN-D-3 TO 25-HYDROXYVITAMIN D-3 AND 1-ALPHA,25-DIHYDROXYVITAMIN D-3

Authors
TAKEDA K ASOU T MATSUDA A KIMURA K OKAMURA K OKAMOTO R SASAKI J ADACHI T OMURA S
Citation
K. Takeda et al., APPLICATION OF CYCLODEXTRIN TO MICROBIAL TRANSFORMATION OF VITAMIN-D-3 TO 25-HYDROXYVITAMIN D-3 AND 1-ALPHA,25-DIHYDROXYVITAMIN D-3, Journal of fermentation and bioengineering, 78(5), 1994, pp. 380-382
Citations number
9
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
78
Issue
5
Year of publication
1994
Pages
380 - 382
Database
ISI
SICI code
0922-338X(1994)78:5<380:AOCTMT>2.0.ZU;2-5
Abstract
Amycolata autotrophica converts vitamin D-3(VD3) to 1 alpha,25-dihydro xyvitamin D-3(1 alpha,25(OH)(2)VD3) via 25-hydroxyvitamin D-3(25(OH)VD 3) by hydroxylation of VD3 at C-25 and C-1. In this microbial hydroxyl ation, it was found that cyclodextrin (CD) had the ability to enhance the hydroxylation of VD3. Addition of partially-methylated-beta-cyclod extrin (PMCD) increased the productivity of 25(OH)VD3 about seven-fold compared to that without CD. Combined use of PMCD and gamma-CD increa sed the production of 1 alpha,25(OH)(2)VD3 in a tank fermenter about s ixteen-fold compared to that without CD.