AH RECEPTOR IN DIFFERENT TISSUES OF C57BL 6J AND DBA/2J MICE - USE OFCOMPETITIVE POLYMERASE CHAIN-REACTION TO MEASURE AH-RECEPTOR MESSENGER-RNA EXPRESSION/
W. Li et al., AH RECEPTOR IN DIFFERENT TISSUES OF C57BL 6J AND DBA/2J MICE - USE OFCOMPETITIVE POLYMERASE CHAIN-REACTION TO MEASURE AH-RECEPTOR MESSENGER-RNA EXPRESSION/, Archives of biochemistry and biophysics, 315(2), 1994, pp. 279-284
A competitive polymerase chain reaction (PCR) method was used to deter
mine the Ah-receptor mRNA content in several tissues of 2,3,7,8-tetrac
hlorodibenzo-p-dioxin (TCDD)-sensitive C57BL/6J and less susceptible D
BA/2J mice. Total RNA from liver, lung, thymus, spleen, heart, kidney,
brain, muscle, and placenta was isolated and reverse-transcribed. Eac
h cDNA was coamplified with a known amount of homologous competitor fr
agment, and PCR products were quantified by extrapolating against a st
andard curve. The Ah-receptor mRNA was expressed in all tissues examin
ed. The highest mRNA level was found in lung, yielding concentrations
of 31.7 +/- 11.0 X 10(3) molelcules/100 ng RNA and 20.3 +/- 8.9 X 10(3
) molecules/100 ng RNA in C57BL/6J and DBA/2J mice, respectively. In h
eart, liver, thymus, brain, and placenta the Ah-receptor mRNA content
was about 5-10 times lower than in lung. Low levels were found in sple
en, kidney, and muscle. Statistical analysis of the data revealed no s
ignificant differences regarding the Ah-receptor mRNA expression betwe
en C57BL/6J and DBA/2J mice. From these results it is suggested that f
actors other than differences in Ah-receptor mRNA content may be respo
nsible for different susceptibility of C57BL/6J and DBA/2J mice toward
TCDD. (C) 1994 Academic Press, Inc.