RADIATION-INDUCED CHEMICAL-TRANSFORMATIONS OF HUMAN SERUM-ALBUMIN IN AQUEOUS-SOLUTIONS

Citation
Nv. Kondakova et al., RADIATION-INDUCED CHEMICAL-TRANSFORMATIONS OF HUMAN SERUM-ALBUMIN IN AQUEOUS-SOLUTIONS, High energy chemistry, 28(6), 1994, pp. 439-445
Citations number
21
Categorie Soggetti
Chemistry Physical
Journal title
ISSN journal
00181439
Volume
28
Issue
6
Year of publication
1994
Pages
439 - 445
Database
ISI
SICI code
0018-1439(1994)28:6<439:RCOHSI>2.0.ZU;2-0
Abstract
A rate constant of the reaction of human serum albumin (HSA) with OH r adicals was determined using the method of competing scavengers. Chang es in the amino acid composition and some biochemical properties of HS A on radiolysis in a berate buffer (pH 7.4) saturated with N2O or O-2 were correlated quantitatively. It was found that the yield of peptide bonds that lost the ability to be cleaved by trypsin is higher than t he total yield of arginine and lysine residues in HSA. The possibility of accumulation of modified HSA in blood upon irradiation was conside red.