INTERGENERIC HYBRIDIZATION BETWEEN BRASSICA-NAPUS AND SINAPIS-PUBESCENS, AND THE CYTOLOGY AND CROSSABILITY OF THEIR PROGENIES

The cytological possibility of gene transfer from Sinapis pubescens to Brassica napus was investigated. Intergeneric hybrids between Brassic a napus (2n = 38) and Sinapis pubescens (2n = 18) were produced throug h ovary culture. The F-1 hybrids were dihaploid and the chromosome con figurations were (0-1)(III) + (2-11)(II) + (5-24)(I). One F-2 plant wi th 38 chromosomes was obtained from open pollination of the F-1 hybrid . Thirty-one seeds were obtained from the backcross of the F-2 plant w ith B. napus. Five out of seven plants had 38 chromosomes, and the pol len stainability ranged from 0% to 81.4%. In the B-2 plants obtained f rom the backcross of B, plants with B. napus, 66.7% of the plants exam ined had 38 chromosomes. S. pubescens may become a gene source for the improvement of B. napus.