M. Kitakaze et al., EVIDENCE FOR DEACTIVATION OF BOTH ECTOSOLIC AND CYTOSOLIC 5'-NUCLEOTIDASE BY ADENOSINE A(1), RECEPTOR ACTIVATION IN THE RAT CARDIOMYOCYTES, The Journal of clinical investigation, 94(6), 1994, pp. 2451-2456
Adenosine, an important regulator of many cardiac functions, is produc
ed by ectosolic and cytosolic 5'-nucleotidase. The activity of these e
nzymes is influenced by several ischemia-sensitive metabolic factors,
e.g., ATP, ADP, H+, and inorganic phosphate. However, there is no clea
r evidence that adenosine itself affects 5'-nucleotidase activity. Thi
s study tested whether adenosine decreases the activity of ectosolic a
nd cytosolic 5'-nucleotidase. Cardiomyocytes were isolated from adult
male Wistar rats and suspended in the modified Hepes-Tyrode buffer sol
ution. After stabilization, isolated cardiomyocytes were incubated wit
h and without adenosine (10(-9)-10(-4) M). Ectosolic and cytosolic 5'-
nucleotidase activity was decreased by exogenous adenosine (ectosolic
5'-nucleotidase activity, 20.6+/-2.3 vs. 8.6+/-1.6 mu mol/min per 10(6
) cells [P < 0.05]; cytosolic 5'-nucleotidase activity, 2.47+/-0.58 vs
. 1.61+/-0.54 mu mol/min per 10(6) cells [P < 0.05] at 10(-6) M adenos
ine) after 30 min. The decrease in ectosolic and cytosolic 5'-nucleoti
dase activity was inhibited by 8-phenyltheophylline and pertussis toxi
n, and was mimicked by N-6-cyclohexyladenosine, an adenosine A(1) rece
ptor agonist. Neither CGS21680C, and A(2) receptor agonist, nor cycloh
eximide deactivated ectosolic and cytosolic 5'-nucleotidase. Thus, we
conclude that activation of adenosine A(1) receptors is coupled to G(i
) proteins and attenuates ectosolic and cytosolic 5'-nucleotidase acti
vity in rat cardiomyocytes.