E. Hamsikova et al., PRESENCE OF ANTIBODIES TO 7 HUMAN PAPILLOMAVIRUS TYPE-16-DERIVED PEPTIDES IN CERVICAL-CANCER PATIENTS AND HEALTHY CONTROLS, The Journal of infectious diseases, 170(6), 1994, pp. 1424-1431
Sera from 133 cervical cancer patients and 154 healthy women (controls
) from Spain and Colombia were tested in IgG-specific ELISAs for the p
resence of antibodies against seven peptides derived from five open-re
ading frames of human papillomavirus (HPV) type 16. Three of the pepti
des corresponded to overlapping regions of the N-terminal half of E7 p
rotein; the other peptides corresponded to selected regions of E2, E4,
L1, and L2 proteins. The prevalence of antibodies against E2 and E7 p
eptides was significantly different between patients and controls. The
most marked differences were for E7 peptides. HPV DNA polymerase chai
n reaction diagnoses of cervical scrapes were available; these were co
rrelated with serologic findings. In HPV-16 DNA-positive patients, E7
antibodies were more broadly and more strongly reactive than in other
patient groups.