EXPRESSION OF CYTOCHROME-P-450 3A IN HT29-MTX CELLS AND CACO-2 CLONE TC7

Authors
CARRIERE V LESUFFLEUR T BARBAT A ROUSSET M DUSSAULX E COSTET P DEWAZIERS I BEAUNE P ZWEIBAUM A
Citation
V. Carriere et al., EXPRESSION OF CYTOCHROME-P-450 3A IN HT29-MTX CELLS AND CACO-2 CLONE TC7, FEBS letters, 355(3), 1994, pp. 247-250
Citations number
35
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
355
Issue
3
Year of publication
1994
Pages
247 - 250
Database
ISI
SICI code
0014-5793(1994)355:3<247:EOC3IH>2.0.ZU;2-9
Abstract
HT-29 sublines and Caco-2 clones were analyzed for the expression of c ytochrome P-450 3A. The enzyme was found to be expressed in differenti ated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, ar e intermediate between those observed in human adult intestine and fet al liver.