CLONING OF THE RAT AND HUMAN PROSTAGLANDIN-F2-ALPHA RECEPTORS AND THEEXPRESSION OF THE RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR

We have cloned the FP receptor from rat corpus luteum and human uterus cDNA libraries, respectively. The coding DNA sequence in the rat cDNA is 1101 bp and is similar to the mouse cDNA coding for a receptor pro tein of 366 amino acids. The human sequence shows a 5 bp deficiency in the 3' region, truncating the coding sequence to 359 amino acids. Nor thern blot analysis indicates highest expression in the ovary. Cell li nes have been established giving stable expression of the FP receptor. Activation of the cloned FP receptor gave an increase in intracellula r calcium, indicating signaling via phospholipase C-mediated phosphoin ositide turnover. Using [H-3]PGF(2 alpha), binding of PGs showed the r ank order of fluprostenol > PhXA70 > PGF(2 alpha) greater than or equa l to PhXA85 > PGD(2) > PGE(2).