MODIFICATION OF 4-BROMOMETHYL-7-METHOXYCOUMARIN DERIVATIZATION METHODOF FATTY-ACIDS FOR HPLC ANALYSIS

Authors
SLEBIODA M PAZDRO K LEWANDOWSKA J FALKOWSKI L
Citation
M. Slebioda et al., MODIFICATION OF 4-BROMOMETHYL-7-METHOXYCOUMARIN DERIVATIZATION METHODOF FATTY-ACIDS FOR HPLC ANALYSIS, Chemia Analityczna, 39(4), 1994, pp. 439-443
Citations number
6
Categorie Soggetti
Chemistry Analytical
Journal title
Chemia Analityczna → ACNP
ISSN journal
00092223
Volume
39
Issue
4
Year of publication
1994
Pages
439 - 443
Database
ISI
SICI code
0009-2223(1994)39:4<439:MO4DM>2.0.ZU;2-L
Abstract
Several parameters affecting formation of fatty acid 4-methyl-7-methox ycoumarin esters have been investigated. The reaction time, temperatur e, concentration and the nature of the basic reagent were varied to ob tain good yield of the derivatives. 18-Crown-6/K2CO3 phase transfer ca talyst was successfully substituted with tetraethylammonium hydroxide. The modified method of the fatty acids labelling gave good reproducib ility (below 6% RSD at nmole level) and detection limit.