ELECTRONIC-PROPERTIES OF THE DISSIMILATORY SULFITE REDUCTASE FROM DESULFOVIBRIO-VULGARIS (HILDENBOROUGH) - COMPARATIVE-STUDIES OF OPTICAL-SPECTRA AND RELATIVE REDUCTION POTENTIALS FOR THE [FE4S4]-SIROHAEM PROSTHETIC CENTERS
Sm. Lui et al., ELECTRONIC-PROPERTIES OF THE DISSIMILATORY SULFITE REDUCTASE FROM DESULFOVIBRIO-VULGARIS (HILDENBOROUGH) - COMPARATIVE-STUDIES OF OPTICAL-SPECTRA AND RELATIVE REDUCTION POTENTIALS FOR THE [FE4S4]-SIROHAEM PROSTHETIC CENTERS, Biochemical journal, 304, 1994, pp. 441-447
The dissimilatory sulphite reductase (desulfoviridin) from the sulphat
e-reducing bacterium Desulfovibrio vulgaris (Hildenborough) displays d
istinct optical and redox characteristics relative to the haem subunit
of Escherichia coli assimilatory sulphite reductase. For high-spin pe
ntaco-ordinate desulfoviridin there is minimal change in the absorbanc
e of the oxidized chromophores both after reduction or after addition
of exogenous ligands. A ligand-metal charge-transfer band similar to 7
02 nm is observed in both the oxidized and one-electron-reduced enzyme
. E.p.r. spectroscopy has been used to define the relative reduction p
otentials for sirohaem and [Fe4S4] centres (Delta E(0) = E(s)(0) - E(c
)(0)) as a function of sirohaem axial co-ordination. Typically Delta E
(0) lies in a range from - 10 to - 50 mV. These results show a correla
tion with the sigma-donor or pi-acceptor properties of the ligand and
stand in sharp contrast with estimates for the E. coli enzyme. The ele
ctronic properties of the coupled [Fe4S4]sirohaem redox centre common
to both nitrite- and sulphite-reducing enzymes are apparently strongly
dependent on the environment generated by protein side chains.