DETERMINATION OF FREE MALONALDEHYDE FORMED IN LIVER-MICROSOMES UPON CCL4 OXIDATION

Free malonaldehyde formed in the microsomes prepared from livers of mo nkey, rat, rabbit, mouse, cow, pig, dog, sheep and horse upon CCl4 oxi dation was derivatized by reaction with N-methylhydrazine to form 1-me thylpyrazole which was subsequently analyzed by capillary gas chromato graphy. Among the livers from animals tested, the monkey and rat liver s produced the most malonaldehyde upon CCl4 treatment. Horse liver sho wed the greatest resistance to CCl4 oxidation. The gas chromatography method used in the present study exhibited an accurate and specific me asurement of free malonaldehyde that might provide an understanding of the biochemical process of in vitro lipid peroxidation.