HIGH-RESOLUTION SEPARATION OF PCR PRODUCT AND GENE DIAGNOSIS BY CAPILLARY GEL-ELECTROPHORESIS

High-resolution separation of a PCR product from a mixture of DNA rest riction fragments was achieved using capillary gel electrophoresis. Th e capillary gel electrophoretic separation gives an excellent resoluti on of two fragments of the 500-bp PCR product and the 506-bp DNA fragm ent, which differs by only 6 bp, and the complete separation of a broa der chain length range of DNA afragments up to 12 kbp within 20 min. T he plate number of gel-filled capillary was achieved to be 2 million p lates per meter. Capillary gel electrophoresis is applied to the gene diagnosis for heart disease through apolipoprotein E genotyping. The a dvantages and limitations of capillary gel electrophoresis in the appl ication to PCR analysis and DNA diagnosis are discussed.