SIMULTANEOUS DETERMINATION OF P-AMINOBENZOIC ACID AND ITS METABOLITESIN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

We developed a new HPLC method for the determination of p-aminobenzoic acid (PABA) and its metabolites (p-aminohippuric acid, N-acetyl-p-ami nohippuric acid, N-acetyl-p-aminobenzoic acid) in urine. As the intern al standard m-hydroxybenzoic acid was used. In the isocratic elution t he mobile phase consisted of methanol and 0.02 M ammonium acetate (20: 80 v/v, pH 4.0). The separation was carried out on the C-18, reversed- phase column, particle size 5 mu m. The separated components were dete cted at 280 nm. The method can be used in the assessment of the respon se of pancrease (secretion of digestive enzymes) to soya feeding as we ll as in the diagnosis of the exocrine pancreatic diseases of animals.