Mt. Lima et al., NEGATIVE TISSUE PARASITISM IN MICE INJECTED WITH A NONINFECTIVE CLONEOF TRYPANOSOMA-CRUZI, Parasitology research, 81(1), 1995, pp. 6-12
Trypanosoma cruzi is a heterogeneous population of parasites as shown
by differences between strains and cloned stock from the same strain.
Herein we present evidence of the noninfectivity of CL-14, a clone der
ived from the CL strain of T. cruzi. In a previous paper we reported t
he absence of parasitemia and mortality in mice injected with metacycl
ic trypomastigotes of this clone. To investigate further this lack of
infectivity we did and extensive histopathological analysis in mice at
different intervals after i.p. (5 and 15 days as well as 1, 4, and 12
months) or i.v. (5 and 30 days) injection of trypomastigotes. In spit
e of a systematic search in all tissues and organs of the animals, no
parasite or significant pathological change was detected in any of the
tissue sections. These data suggest the inability of this clone to me
diate infection and/or cause pathological alterations in vivo.