M. Nakajima et al., AN ONLINE ADVISORY CONTROL-SYSTEM FOR THE LACTIC-ACID FERMENTATION PROCESS, Applied microbiology and biotechnology, 42(2-3), 1994, pp. 204-211
A fuzzy expert system was developed for online diagnosing and controll
ing of bioprocesses. The system was constructed in object-oriented Sma
lltalk/V for diagnosing and controlling of bioprocesses. Lactic acid f
ermentation with an industrial strain of Lactobacillus casei was chose
n as the model system. The performance of the fuzzy expert system and
the knowledge base utilizing experts' knowledge and several facts obta
ined from the experiments were successfully validated with on-line fer
mentations. The fuzzy expert system could diagnose a fault on-line and
give reasonable advice to the process operator. In order to achieve t
he diagnosing faculty, a database, a knowledge base, and both backward
and forward chaining procedures were implemented employing the object
-oriented programming environment. A defuzzifier was implemented in th
e system to achieve on-line control. In order to realize a decision-ma
king system with a human operator and a fuzzy expert system, a new con
trol strategy named Advice was also introduced. Several cultivations w
ere carried out in order to collect knowledge on the effects concerned
with inoculum properties to the process and to construct a database i
ncluding standard time-course profiles. The performance of the fuzzy e
xpert control system was successfully tested with on-line experiments.