ANALYSIS OF ISOMERS OF CHOLESTERYL ACETATE HYDROPEROXIDES BY HPLC ANDGC-ITDMS

Gas chromatographic, high performance liquid chromatographic and GC-io n trap detector mass spectrometric analyses of the hydroperoxides form ed during thermal peroxidation of cholesteryl acetate, are reported an d discussed. The hydroperoxides were separated from the thermal-oxidis ed mixture by solid-phase extraction, fractionated and individually co llected by HPLC. Each hydroperoxide was then chemically reduced with N aBH4 to a hydroxy-derivative. The GC and MS characteristics of hydroxy - and TMS-derivatives were compared against available standards. Only 7 beta-hydroperoxide and 7 alpha-hydro-peroxide of cholesteryl acetate were found, as reported in the literature for cholesterol.