Nq. Bai et al., DESIGN OF A CRYOGENIC VIDEOCAMERA-RECORDER AND IMAGE-PROCESSING SYSTEM AND CALCULATION OF VOLUMES OF RED-CELLS, Cryobiology, 31(6), 1994, pp. 549-556
Since Rowe reported that the storage technique for red cells at very l
ow temperatures had been realized successfully (4, 5), many experts wh
o work in the fields of cryobiology and medicine have turned their att
ention to this storage technique for tissues and organs (3). Since the
first quantitative cryomicroscope was made successfully about 20 year
s ago (1), it has been possible to observe changes in shape and phase.
Particularly the image processing technique has laid the foundation f
or quantitative analysis of the relationship between changes in shape
and damage from freezing of cells and cooling rate and storage tempera
ture. In this work, we constructed a system consisting of a cryomicros
cope, a videocamera-recorder, and an image processor. We carried out m
any experiments with red cells in cold storage and have established a
model for calculating the volumes of red cells. In our experiments we
also dynamically traced the shape changes of cells with various experi
mental parameters. (C) 1994 Academic Press, Inc.