DESIGN OF A CRYOGENIC VIDEOCAMERA-RECORDER AND IMAGE-PROCESSING SYSTEM AND CALCULATION OF VOLUMES OF RED-CELLS

Citation
Nq. Bai et al., DESIGN OF A CRYOGENIC VIDEOCAMERA-RECORDER AND IMAGE-PROCESSING SYSTEM AND CALCULATION OF VOLUMES OF RED-CELLS, Cryobiology, 31(6), 1994, pp. 549-556
Citations number
8
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
ISSN journal
00112240
Volume
31
Issue
6
Year of publication
1994
Pages
549 - 556
Database
ISI
SICI code
0011-2240(1994)31:6<549:DOACVA>2.0.ZU;2-H
Abstract
Since Rowe reported that the storage technique for red cells at very l ow temperatures had been realized successfully (4, 5), many experts wh o work in the fields of cryobiology and medicine have turned their att ention to this storage technique for tissues and organs (3). Since the first quantitative cryomicroscope was made successfully about 20 year s ago (1), it has been possible to observe changes in shape and phase. Particularly the image processing technique has laid the foundation f or quantitative analysis of the relationship between changes in shape and damage from freezing of cells and cooling rate and storage tempera ture. In this work, we constructed a system consisting of a cryomicros cope, a videocamera-recorder, and an image processor. We carried out m any experiments with red cells in cold storage and have established a model for calculating the volumes of red cells. In our experiments we also dynamically traced the shape changes of cells with various experi mental parameters. (C) 1994 Academic Press, Inc.