Lc. Waters et al., EVALUATION OF 2 FIELD SCREENING-TEST KITS FOR THE DETECTION OF PCBS IN SOIL BY IMMUNOASSAY, Journal of AOAC International, 77(6), 1994, pp. 1664-1671
Effective field screening methods could minimize the time and reduce t
he cost of characterizing and remediating hazardous waste sites. Rigor
ous evaluation of novel field screening methods is required before the
y can be considered as replacements for, or adjuncts to, currently use
d laboratory methods. Alternatives to standard laboratory analytical m
ethods should be rapid, analyte-specific, cost-effective, accurate, an
d sensitive in the range at which the analyte is regulated. In this st
udy, 2 immunoassay-based field test kits for polychlorinated biphenyls
(PCBs) in soil were evaluated with reference to those criteria. PCBs
were analyzed in both spiked and field soil samples. Based on laborato
ry performance, we estimate that 20 to 40 samples can be analyzed in t
he field per day. Sensitivity of the assay is in the 1 ppm range. Beca
use the assay is based on the specificity of the antigen/antibody reac
tion, interferences are practically negligible. The method is accurate
; the false-negative and false-positive results that were observed can
be explained by differences in the immunoreactivities of the Aroclors
present in the test samples and the Aroclors used as standards in the
assay. The savings in time and expense to analyze PCBs in soil with t
he immunoassay-based test kits over conventional laboratory methods sh
ould be substantial.