CYCLIC CMP FORMATION IN RAT CEREBELLAR SLICES IS STIMULATED BY ENDOTHELINS VIA NITRIC-OXIDE FORMATION AND BY SARAFOTOXINS VIA FORMATION OF CARBON-MONOXIDE
Z. Shragalevine et al., CYCLIC CMP FORMATION IN RAT CEREBELLAR SLICES IS STIMULATED BY ENDOTHELINS VIA NITRIC-OXIDE FORMATION AND BY SARAFOTOXINS VIA FORMATION OF CARBON-MONOXIDE, Biochemistry, 33(49), 1994, pp. 14656-14659
Involvement of a cyclic GMP pathway in signal transduction stimulated
by endothelins (ETs) and sarafotoxins (SRTXs) was explored using rat c
erebellar slices. These peptides activated the same receptor binding s
ites (ET-1 and SRTX-b at the picomolar sites; ET-3 and SRTX-c at the n
anomolar sites) to produce cyclic GMP, but their signaling pathways di
ffered. The endothelins (ET-1 and ET-3) were found to signal via nitri
c oxide formation and to involve pertussis toxin-sensitive G-protein(s
), The SRTXs (b and c), while also stimulating cyclic GMP production,
did so via a pathway which is not L-arginine-dependent, i.e., carbon m
onoxide formation, and did not involve pertussis-toxin-sensitive G-pro
tein(s). This is the first demonstration that the signaling pathways o
f endothelins and sarafotoxins may differ, even though they share the
same binding sites.