S. Greasley et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON ADP-RIBOSYLATION FACTOR 1, Journal of Molecular Biology, 244(5), 1994, pp. 651-653
ADP-ribosylation factor 1 (ARF-1) is a member of a family of small G-p
roteins that regulate both intracellular vesicle transport and phospho
lipase D activity Crystals of ARF-1 suitable for X-ray diffraction ana
lysis have been grown in the presence of GDP by the hanging drop vapou
r diffusion method. Crystals grow in space group C2 with cell dimensio
ns a = 122.36 Angstrom, b = 45.01 Angstrom, 91.96 Angstrom and beta =
133.62 degrees and diffract to at least 2.3 Angstrom resolution. A sec
ond crystal form has been characterized (space group C2, a = 69.70 Ang
strom, b = 45.25 Angstrom, c = 60.45 Angstrom, beta = 109.6 degrees) b
ut does not grow reproducibly.