J. Zhou et L. Fritz, OKADAIC ACID ANTIBODY LOCALIZES TO CHLOROPLASTS IN THE DSP-TOXIN-PRODUCING DINOFLAGELLATES PROROCENTRUM-LIMA AND PROROCENTRUM-MACULOSUM, Phycologia, 33(6), 1994, pp. 455-461
Thin sections of Prorocentrum lima (Ehrenberg) Dodge and Prorocentrum
maculosum Faust, two dinoflagellates known to produce diarrhetic shell
fish poisoning (DSP), were probed with a monoclonal antibody targeted
against okadaic acid (OA), the primary toxin in DSP outbreaks. The ant
ibody localizes primarily to chloroplasts and pyrenoids and, to a less
er degree, cellular lysosomes. Controls on the primary antibody lack l
abelling as do controls done on a non-producer of toxin, Prorocentrum
micans Ehrenberg, and a paralytic shellfish poison (PSP) producer, Ale
xandrium tamarense (Lebour) Balech. OA is likely to be produced by the
chloroplasts of the cell and its compartmentalization in plastids may
explain why producer cells are unaffected by the toxicity of OA to eu
karyotic cytoplasmic phosphatases.