BACTERIOCIN PRODUCTION BY ENTEROCOCCUS-FAECIUM NA01 FROM WARA - A FERMENTED SKIMMED COW MILK PRODUCT FROM WEST-AFRICA

Authors
OLASUPO NA SCHILLINGER U FRANZ CMAP HOLZAPFEL WH
Citation
Na. Olasupo et al., BACTERIOCIN PRODUCTION BY ENTEROCOCCUS-FAECIUM NA01 FROM WARA - A FERMENTED SKIMMED COW MILK PRODUCT FROM WEST-AFRICA, Letters in applied microbiology, 19(6), 1994, pp. 438-441
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
19
Issue
6
Year of publication
1994
Pages
438 - 441
Database
ISI
SICI code
0266-8254(1994)19:6<438:BPBENF>2.0.ZU;2-S
Abstract
An Enterococcus faecium strain from Nigerian fermented skimmed cow mil k ('wara') produced bacteriocin inhibitory towards Lactobacillus, Ente rococcus and Listeria strains. The bacteriocin (designated enterocin 0 1) was inactivated by proteases, heat-stable at 100 degrees C and acti ve at pH 2.0-6.0, The Ent. faecium isolate harboured plasmids of cn 36 .3 and 23.1 kb. Curing experiments with ethidium bromide resulted in a bacteriocin-negative mutant which had not lost immunity to the bacter iocin. Slight differences in plasmid profiles between wild-type and mu tant indicated a possible plasmid-coded bacteriocin production,