ISOLATION AND SELECTION OF AN L-AMINOACYLASE-PRODUCING BACTERIUM, PSEUDOMONAS SP BA2

The enrichment culture method was used to detect and isolate L-aminoac ylase-producing bacteria from soil, using N-acetyl-L-alanine as induce r and substrate. Isolated bacterial strains were screened for growth a nd enzyme activity. Strain BA2 displayed both the highest intracellula r L-aminoacylase activity and the most profuse growth. Furthermore, BA 2 cells did not show any D-aminoacylase activity. This strain was an o bligately aerobic rod-shaped bacterium and stained Gram-negative, and was therefore identified as Pseudomonas. Its morphological and biochem ical characteristics corresponded to those of Pseudomonas fluorescens biovar I.