Ab. Santoyo et al., ISOLATION AND SELECTION OF AN L-AMINOACYLASE-PRODUCING BACTERIUM, PSEUDOMONAS SP BA2, Letters in applied microbiology, 19(6), 1994, pp. 461-465
The enrichment culture method was used to detect and isolate L-aminoac
ylase-producing bacteria from soil, using N-acetyl-L-alanine as induce
r and substrate. Isolated bacterial strains were screened for growth a
nd enzyme activity. Strain BA2 displayed both the highest intracellula
r L-aminoacylase activity and the most profuse growth. Furthermore, BA
2 cells did not show any D-aminoacylase activity. This strain was an o
bligately aerobic rod-shaped bacterium and stained Gram-negative, and
was therefore identified as Pseudomonas. Its morphological and biochem
ical characteristics corresponded to those of Pseudomonas fluorescens
biovar I.