YEAST ARTIFICIAL CHROMOSOME AND RADIATION HYBRID MAP OF LOCI IN CHROMOSOME BAND 8P22, A COMMON REGION OF ALLELIC LOSS IN MULTIPLE HUMAN CANCERS

Polymorphic alleles at loci such as LPL (lipoprotein lipase) and MSR ( macrophage scavenger receptor) in chromosome band 8p22 are frequently lost during the genesis of several types of human cancer, including co lorectal, non-small cell lung, hepatocellular, and prostatic carcinoma s. A physical map of 31 published or novel probes and sequence-tagged sites in this genetic region was constructed using a radiation hybrid panel and the CEPH (Centre d'Etude du Polymorphisme Humain) yeast arti ficial chromosome (YAC) library. Thirty-six overlapping YACs defined a physical order for the following polymorphic markers: tel - D8S26 - D 8S511 - D8S549 - ICISR - D8S254 - D8S233 - D8S261 - D8S21 - LPL - D8S2 58 - cen. These maps unify small consensus regions of allelic loss on chromosome 8p defined by restriction fragment length polymorphisms wit h more informative PCR-based polymorphisms and widely available YAC ma pping resources. (C) 1993 Academic Press, Inc.