IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF C-TERMINUS OF 43-KDA DYSTROPHIN-ASSOCIATED GLYCOPROTEIN IN NORMAL HUMAN SKELETAL MYOFIBERS

Ultrastructural localization of the C-terminus of a 43-kDa dystrophin- associated glycoprotein (43-DAG) and its relation to dystrophin were i nvestigated in normal human skeletal myofibers by using single and dou ble immunogold labelling methods. The C-terminal end of 43-DAG antibod y was localized ultrastructurally at the inside surface of muscle plas ma membrane and the sarcoplasmic side of the plasma membrane invaginat ions. Double immunolabelling electron microscopy disclosed that signal s of the C-terminal end of 43-DAG and dystrophin were often associated with each other and formed a doublet at the inside surface of plasma membranes in normal human skeletal myofibers.