RADIONUCLIDE TARGETING AND DOSIMETRY AT THE MICROSCOPIC LEVEL - THE ROLE OF MICROAUTORADIOGRAPHY

Citation
Mrb. Puncher et Pj. Blower, RADIONUCLIDE TARGETING AND DOSIMETRY AT THE MICROSCOPIC LEVEL - THE ROLE OF MICROAUTORADIOGRAPHY, European journal of nuclear medicine, 21(12), 1994, pp. 1347-1365
Citations number
107
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
ISSN journal
03406997
Volume
21
Issue
12
Year of publication
1994
Pages
1347 - 1365
Database
ISI
SICI code
0340-6997(1994)21:12<1347:RTADAT>2.0.ZU;2-B
Abstract
The understanding of localisation mechanisms and microdosimetry of dia gnostic and therapeutic radiopharmaceuticals depends on knowledge of t heir biodistribution at the microscopic level (cellular and subcellula r) in the target tissues. Various methods have been advanced for obtai ning information about this microdistribution: subcellular fractionati on, secondary ion mass spectrometry imaging, microprobe elemental anal ysis in the electron microscope, and microautoradiography. This review compares these approaches, and discusses in detail the methodology of microautoradiography (the most generally useful approach) with imagin g and therapy radionuclides, Literature examples of applications of mi croautoradiography in nuclear medicine are reviewed, and the future po tential contribution of the techniques is assessed.