5'-FLANKING REGION SURROUNDING A HUMAN CYTOSOLIC PHOSPHOLIPASE-A2 GENE

The 5'-flanking region and the first four exons of a gene encoding hum an cytosolic phospholipase Az (cPLA2) were isolated from a human lambd a EMBL3 genomic library and sequenced. The 5'-flanking region is chara cterized by CA repeats, one of microsatellites. The analysis of the 5' -flanking region with transcription factor database suggests the exist ence of the transcription factor binding sites such as NF-KB, NF-IL6, AP-1, AP-2, and PEA3. These factors are well known to be induced or ac tivated by the reagents (tumor necrosis factor alpha, interleukin-1, e pidermal growth factor, and phorbol myristate acetate) reported as the inducers of a cPLA2 gene. (C) 1994 Academic Press, Inc.