STIMULATION OF CELL-MEDIATED LOW-DENSITY-LIPOPROTEIN OXIDATIVE MODIFICATION BY ONCOSTATIN-M

The effect of oncostatin M on low-density lipoprotein oxidative modifi cation by human monocytes or murine endothelial cells was studied by d etermination of the lipid peroxidation products content and the electr ophoretic mobility of the particle: In the range of concentrations fro m 10 to 30 ng/ml, oncostatin M induced a dose-dependent increase in LD L oxidation. The LDL degradation by J774 macrophage-like cells was als o stimulated. Preincubation of cells with oncostatin M also enhanced t he subsequent LDL modification This effect was accompanied by a parall el increase in superoxide anion release by cells. Since oncostatin M i s produced by immune cells, the described effect suggests a relationsh ip between infection, inflammation and LDL oxidative modification. (C) 1994 Academic Press, Inc.