PORCINE LUTEAL CELLS EXPRESS MONOCYTE CHEMOATTRACTANT PROTEIN-2 (MCP-2) - ANALYSIS BY CDNA CLONING AND NORTHERN ANALYSIS

From an expression library in lambda UniZAP, derived from porcine corp us luteum (CL), a clone lambda MCP9 was detected by hybridization with a porcine MCP-1 specific probe. A pBluescriptSK-derivative pMCP9 was generated from lambda LMCP9 by in vivo excision and was shown to conta in an open reading frame (ORF) encoding a protein highly homologous to bovine monocyte chemoattractant protein-2 (MCP-2). Comparison of amin o acid sequences of known MCPs identified the protein encoded by pMCP9 as porcine MPC-2. The 3' untranslated region of pMCP9 was completed b y 3' RACE. Northern analysis using RNA from porcine luteal cells and p robes specific for porcine MCP-1 and MCP-2 revealed that porcine lutea l cells express both MCPs. According to Southern analysis MCB-2, like MCP-1, is specified by a single copy gene. (C) 1994 Academic Press, In c.