CHARACTERIZATION OF THE HUMAN TYPE-2 NEUROPEPTIDE-Y RECEPTOR GENE (NPY2R) AND LOCALIZATION TO THE CHROMOSOME 4Q REGION CONTAINING THE TYPE-1 NEUROPEPTIDE-Y RECEPTOR GENE

Neuropeptide Y (NPY) signals through a family of G-protein coupled rec eptors present in the brain and sympathetic neurons, To further our un derstanding of the genetic elements involved in the regulation of NPY receptor expression, we have cloned and characterized the human gene e ncoding the type 2 NPY receptor (Y2 receptor, HGMW-approved symbol NPY 2R).(2) The transcript spans 9 kb of genomic sequence and is encoded o n two exons. As in the type 1 NPY receptor (YI receptor) gene, the 5'- untranslated region of the Y2 receptor is interrupted by an intervenin g sequence (similar to 4.5 kb), However, the Y2 receptor gene does not contain an intron analogous to that present in the coding region of t he Y1 receptor. The predicted transcript size (similar to 4.5 kb) is c onsistent with the size observed by Northern analysis. The 381-amino-a cid sequence deduced from the open reading frame is identical to that encoded by the cDNA. The Y2 receptor gene maps to human chromosome 4q3 1, the same region containing the Y1 receptor locus, suggesting that t hese subtypes may have arisen by gene duplication despite their struct ural differences. (C) 1996 Academic Press, Inc.