INTRACELLULAR MEDIATORS OF GOBLET CELL DEGRANULATION IN ISOLATED PANCREATIC DUCTS

The involvement of particular intracellular signalling pathways in ago nist-evoked degranulation of guinea pig pancreatic duct goblet cells w as investigated. Carbachol, vasoactive intestinal peptide (VIP), calci um ionophore A23187, phorbol ester 12-O-tetradecanoylphorbol 13-acetat e (TPA),cyclic AMP analogue Sp-5,6-DCl-cBIMPS and forskolin each cause d degranulation of goblet cells in isolated ducts. Degranulation induc ed by carbachol was not inhibited by okadaic acid, cytochalasin-D or n ocodazole. These results indicate that at least two major signalling p athways are involved in pancreatic duct goblet cell secretion. (C) 199 4 Academic Press, Inc.