ISOLATION, SEQUENCING, AND MAPPING OF THE HUMAN HOMOLOG OF THE YEAST TRANSCRIPTION FACTOR, SPT5

We isolated the human homologue, SUPT5H, of the yeast transcription fa ctor, SPT5. The human homologue is 1088 aa long compared to 1063 aa fo r the yeast gene, SUPT5H maps to 19q13, near the ryanodine receptor. L ike its family member, SUPT6H, and like yeast SPT5, SUPT5H has a very acidic 5' domain, Like its family member, SUPT6H, but unlike yeast SPT 5 or SPT6, SUPT5H has seven MAP kinase sites at its 5' end. In additio n, SUPT5H lacks the novel g-amino-acid repeat (consensus is S-T/A-W-G- G-A/Q) at the C-terminus of yeast SPT5. This argues that while there i s functional similarity between SPT5 and SUPT5H, the molecules differ in the signals to which they respond. (C) 1996 Academic Press, Inc.