VASOACTIVE INTESTINAL POLYPEPTIDE STIMULATES CYCLIC-AMP PRODUCTION INMOUSE N1E-115 NEUROBLASTOMA-CELLS - MODULATION BY A PROTEIN-KINASE-C ACTIVATOR AND IONOMYCIN

Authors
INUKAI T CHIK CL HO AK
Citation
T. Inukai et al., VASOACTIVE INTESTINAL POLYPEPTIDE STIMULATES CYCLIC-AMP PRODUCTION INMOUSE N1E-115 NEUROBLASTOMA-CELLS - MODULATION BY A PROTEIN-KINASE-C ACTIVATOR AND IONOMYCIN, Peptides, 15(8), 1994, pp. 1361-1365
Citations number
22
Categorie Soggetti
Biology
Journal title
Peptides → ACNP
ISSN journal
01969781
Volume
15
Issue
8
Year of publication
1994
Pages
1361 - 1365
Database
ISI
SICI code
0196-9781(1994)15:8<1361:VIPSCP>2.0.ZU;2-6
Abstract
In this study, we investigated the vasoactive intestinal polypeptide ( VIP)-stimulated cAMP production and its interaction with protein kinas e C activation and elevation of intracellular Ca2+ in N1E-115 neurobla stoma cells. VIP treatment caused a 55-fold increase in cAMP accumulat ion. Addition of 4 beta-phorbol 12-myristate 13-acetate reduced VIP- b ut not forskolin-stimulated cAMP response. In comparison, ionomycin po tentiated both VIF- and forskolin-induced cAMP accumulation. Our resul ts indicate that VIP stimulates cAMP accumulation in N1E-115 cells, an d that although activation of protein kinase C inhibits the VIP-stimul ated cAMP response, elevation of intracellular Ca2+ potentiates this s ignaling pathway.