W. Krenger et al., EFFECTS OF EXOGENOUS INTERLEUKIN-10 IN A MURINE MODEL OF GRAFT-VERSUS-HOST DISEASE TO MINOR HISTOCOMPATIBILITY ANTIGENS, Transplantation, 58(11), 1994, pp. 1251-1257
IL-10 is a regulatory cytokine of both T cells and monocytes. We have
investigated the ability of IL-10 to regulate responses to alloantigen
s in vitro and in vivo. Addition of IL-10 to mixed lymphocyte cultures
profoundly decreased the proliferation and IL-2 production by donor B
10.BR cells stimulated with CBA cells expressing minor histocompatibil
ity antigens. Administration of IL-10 for a period of 2 weeks after bo
ne marrow transplantation decreased the expansion of CD4(+) and CD8(+)
donor T cells. In addition, splenocytes from BMT mice treated with IL
-10 secreted less IFN-gamma after stimulation with Con A in vitro. The
suppression of the mitogen-driven proliferative response of lymphocyt
es from the IL-10-treated group could also be reversed with significan
tly less anti-IFN-gamma antibody than for saline-treated controls. How
ever, treatment with IL-10 was not sufficient to alter significantly t
he clinical course of graft-versus-host disease in CBA recipient mice
as assessed by survival, weight loss, and splenomegaly. The results su
ggest that exogenous IL-10 suppresses the afferent Th1 response in a g
raft-versus-host reaction but does not significantly diminish the effe
ctor stage of graft-versus-host disease.