FUNCTIONAL-ANALYSIS OF THE 5'-FLANKING REGION OF GENE 6B FROM TL-DNA PTIBO542 IN TRANSGENIC TOBACCO PLANTS

Authors
BAGYAN IL REVENKOVA EV KRAEV AS SKRYABIN KG
Citation
Il. Bagyan et al., FUNCTIONAL-ANALYSIS OF THE 5'-FLANKING REGION OF GENE 6B FROM TL-DNA PTIBO542 IN TRANSGENIC TOBACCO PLANTS, Molecular biology, 28(4), 1994, pp. 487-492
Citations number
44
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
28
Issue
4
Year of publication
1994
Part
1
Pages
487 - 492
Database
ISI
SICI code
0026-8933(1994)28:4<487:FOT5RO>2.0.ZU;2-1
Abstract
The activity of P-glucuronidase (GUS; EC 3.2.1.31) was measured in lea ves and roots of transgenic tobacco plants carrying the reporter gene encoding GUS. This gene was controlled by a 826-bp (from translation s tart) fragment of the 5'-flanking region of gene 6b from TL-DNA of pTi Bo542. The GUS activity in roots of the 6b-GUS plants was 20-80 times higher than in leaves. The activity of 5'-flanking region of gene 6b w as stimulated by wounding and by phytohormones.