SEQUENCING EFFICACY OF CONTIGUOUS STACKING HYBRIDIZATION ON OLIGONUCLEOTIDE MATRICES OF VARIABLE TUPLE LENGTH

DNA sequencing efficacy can be enhanced through extending the duplex l ength whereby L-oligonucleotides are added in solution to a matrix of immobilized L-oligonucleotides. The reconstruction efficacy has been e stimated for fragments up to 30,000 units, considering various L+l com binations. The results obtained allow an assessment of the potentialit ies of the proposed technique with various matrices, as well as of the experimental labor involved. Contiguous stacking hybridization substa ntially increases the length of DNA fragments that can be sequenced. A n approach is proposed to solve ambiguities at branch points caused by long repeats, using contiguous stacking hybridization to form a conca tenation of several added oligonucleotides stabilized by pair stacking interactions.