MEASUREMENT OF DISTANCES BETWEEN DNA SEGMENTS INCREASES THE EFFICIENCY OF SEQUENCING BY HYBRIDIZATION WITH OLIGONUCLEOTIDE MATRIX

DNA sequencing by hybridization with a matrix of immobilized oligonucl eotides is not directly is not directly applicable for fragments with long monotonous repeats. Auxiliary information for this purpose can be obtained from the distances between certain segments within the DNA f ragment examined, using DNA restriction or PCR with defined primers fo llowed by measuring the length of the resulting segments in gel electr ophoresis. Such additional information increases the reconstruction ef ficiency and in many cases solves the problem of repeating and monoton ous segments. The work demonstrates the use and appraises the usefulne ss of this information.