REGULATION OF ENZYME-ACTIVITY IN ADSORPTIVE SYSTEMS .5. SLOW CONFORMATIONAL CHANGE IN ENZYME ON ADSORPTION

Kinetic models of adsorptive enzyme systems involving slow conformatio nal changes in the enzyme molecule were considered. The rate of revers ible adsorption of a monomeric or oligomeric enzyme was assumed to be low in comparison with the rate of the enzymic reaction. A model with slow isomerization of the enzyme bound to the adsorbent containing spe cific enzyme binding sites was also analyzed. Equations describing the kinetics of the adsorptive enzyme systems have been obtained for the casks when the substrate-enzyme binding steps are in rapid equilibrium and when the rates of these steps are comparable to that of the catal ytic breakdown of the enzyme-substrate complex. Conditions were specif ied under which the kinetic curves for product accumulation had a lag period or an initial burst.