CHARACTERIZATION OF THO MOUSE MONOCLONAL-ANTIBODY TJ4C4 DIRECTED AT HUMAN P68 KINASE

This report describes the production and characterization of a mouse m onoclonal antibody (mAb) TJ4C4, which is directed against the interfer on-induced double-stranded RNA-activated protein kinase p68 (PKR). The mAb TJ4C4 was produced against p68 which was isolated using a partial ly purified p68 preparation from human cells. The specificity of this mAb is demonstrated in competitive inhibition assays using recombinant ly produced p68 protein and by immunoprecipitation. This mAb is of par ticular value in that it detects an epitope on p68 which is not destro yed in routinely prepared, formalin-fixed paraffin-embedded tissues, o r in a variety of other commonly used clinical fixatives. The inhibiti on of mAb TJ4C4 by recombinantly produced p68, on human tissues sectio ns, validates the specificity of this mAb in histological studies. The refore, mAb TJ4C4 serves as a specific probe to study p68 expression i n clinically derived tissue specimens.